Xuepei Zhang, Massimiliano Gaetani, Alexey Chernobrovkin, and Roman A. Zubarev
The antiproliferation effect of deuterium depleted water (DDW) is well documented, but the mechanism remained elusive. Here three complementary proteomics approaches applied to A549 cells revealed a disbalance brought about by DDW in mitochondria between ROS production and neutralization, thus
leading to oxidative stress in the cells. Subsequent validation by orthogonal approaches supported this scenario. Therefore, DDW has potential as an adjuvant in antitumor therapy, especially in the modalities inducing oxidative stress in cancer cells.